SAE1/SAE2 is the heterodimeric E1 enzyme that conjugates SUMO to protein substrates. The conjugation process is very similar to that of protein ubiquitination. SUMO is conjugated onto the catalytic cysteine residue of SAE2 by formation of a thioester bond in an ATP-dependent reaction. The activated SUMO is then passed on to an E2 (Ubc9) to be finally attached to a substrate.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

6xHis-SAE1/SAE2

SAE1 (AOS1; SUA1; UBLE1A; HSPC140; FLJ3091); SAE2 (RBBP8; RIM; CTIP; JWDS; SCKL2)

B1301

100 µg

SAE1 (33 kDa), SAE2 (71.2 kDa)

Human

Bacterial recombinant

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

~95% by SDS-PAGE, see datasheet for gel image

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Datasheet， MSDS

NM_005500 (SAE1); NM_002894 (SAE2)

(Click image to enlarge)

Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified 6xHis-SAE1/SAE2

Dry ice shipping

1. Desterro JM, et al. (1999) J Biol Chem 274(15), 10618 – 10624.2. Melchior F (2000) Annu Rev Cell Dev Biol 16, 591 – 626.3. Schulman BA, et al. (2009) Nat Rev Mol Biol 10(5), 319 – 331.

Details

SAE1/SAE2 is the heterodimeric E1 enzyme that conjugates SUMO to protein substrates. The conjugation process is very similar to that of protein ubiquitination. SUMO is conjugated onto the catalytic cysteine residue of SAE2 by formation of a thioester bond in an ATP-dependent reaction. The activated SUMO is then passed on to an E2 (Ubc9) to be finally attached to a substrate. Images:(Click image to enlarge) Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified 6xHis-SAE1/SAE2