TheH-XStablePrestainedProteinLadderisathree-colorproteinstandardwith12pre-stainedproteinscoveringawiderangemolecularweightsfrom10to245kDa.Proteinsarecovalentlycoupledwithabluechromophoreexceptfortworeferencebands(onegreenandoneredbandat25kDaand75kDarespectively)whenseparatedonSDS-PAGE(Tris-glycinebuffer).TheH-XStablePrestainedProteinLadderisdesignedformonitoringproteinseparationduringSDS-polyacrylamidegelelectrophoresis,verificationofWesterntransferefficiencyonmembranes(PVDF,nylon,ornitrocellulose)andforapproximatingthesizeofproteins.Theladderissuppliedingello
ADIngbufferandisreadytouse.
AdditionalInformation
ProductName: | H-XStablePrestainedProteinLadder |
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CatalogNo.: | L2020 |
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Size: | 500µl |
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Range: | ~10kDato~245kDa |
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NumberofBands: | 12 |
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Concentration: | Approximately0.1~0.4mg/mlofeachproteininthebuffer(20mMTris-phosphate,pH7.5at25°C),2%SDS,0.2mMDithiothreitol,3.6MUrea,and15%(v/v)Glycerol). |
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RecommendedLoad: | •3μlor5μlperloadingforclearvisualizationduringelectrophoresison15-wellor10-wellmini-gel,respectively •2~3μlperwellforgeneralWesterntransfering. •Applymoreforthicker(>1.5mm)orlargergel. |
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StABIlity: | Stableforupto2weeksat25°C Stableforupto3monthsat4°C Forlongtermstorage,storeat-20°C(Recommended) |
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PDFDataSheet: | PDFDatasheet |
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Image(s): | (Clickimagetoenlarge)
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ShippingMethod: | Roomtemperatureshipping |
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Details
TheH-XStablePrestainedProteinLadderisathree-colorproteinstandardwith12pre-stainedproteinscoveringawiderangemolecularweightsfrom10to245kDa.Proteinsarecovalentlycoupledwithabluechromophoreexceptfortworeferencebands(onegreenandoneredbandat25kDaand75kDarespectively)whenseparatedonSDS-PAGE(Tris-glycinebuffer).TheH-XStablePrestainedProteinLadderisdesignedformonitoringproteinseparationduringSDS-polyacrylamidegelelectrophoresis,verificationofWesterntransferefficiencyonmembranes(PVDF,nylon,ornitrocellulose)andforapproximatingthesizeofproteins.Theladderissuppliedingelloadingbufferandisreadytouse.
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