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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ubpbio/N-terminal Biotin-Ubiquitin/E1140/50 µg
产品编号:E1140
市  场 价:¥2900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$145.00
品      牌: UBPBio
公司分类:
ubpbio/N-terminal Biotin-Ubiquitin/E1140/50 µg
商品介绍
AsinglebiotinmoietyisconjugatedontheN-terminusofubiquitin.Alllysineresiduesofubiquitinarestillavailableforformationofpolyubiquitinchains.TheextremelyhighbindingaffinitybetweenbiotinandstreptavidinallowstheuseofN-terminalbiotin-ubiquitinforthefollowinginvitroassays:1)rapidandefficientpurificationofpolyubiquitinatedsubstrateproteins;2)monitoringsubstrateubiquitinationusinganHRP-conjugatedstreptavidinantibody;and3)settinguphighthroughputFRETassaystomonitorsubstrateubiquitinationinassaysscreeningE3ubiquitinligaseinhibitors.

AdditionalInformation

Details

AsinglebiotinmoietyisconjugatedontheN-terminusofubiquitin.Alllysineresiduesofubiquitinarestillavailableforformationofpolyubiquitinchains.TheextremelyhighbindingaffinitybetweenbiotinandstreptavidinallowstheuseofN-terminalbiotin-ubiquitinforthefollowinginvitroassays:1)rapidandefficientpurificationofpolyubiquitinatedsubstrateproteins;2)monitoringsubstrateubiquitinationusinganHRP-conjugatedstreptavidinantibody;and3)settinguphighthroughputFRETassaystomonitorsubstrateubiquitinationinassaysscreeningE3ubiquitinligaseinhibitors.

Use
Dependingonubiquitinationassayconditions,N-terminalbiotin-ubiquitincanusedinconjunctionwithwildtypeubiquitinatcombinedconcentrationrangeof20-50μMwitha1:4to1:20ratioofN-terminalbiotin-Ub:wildtypeubiquitin.Images:(Clickimagetoenlarge)
ProductName:N-terminalBiotin-Ubiquitin
AlsoKnownAs:Biotin-Ub
CatalogNo.:E1140
Size:50µg
MolecularWeight:8.9kDa
Species:Human
Source:Synthetic
Stock:20mMTris,pH7.6at4°C,100mMNaCl,10%glycerol
Concentration:1mg/mlinaqueousbuffer
QualityAssurance:>95%byHPLC
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFDatasheetMSDS
NCBIRefSeq:N/A
Image(s):(Clickimagetoenlarge)
LC-MSofN-terminalBiotin-Ubiquitin
ShippingMethod:Dryiceshipping
References:N/A
LC-MSofN-terminalBiotin-Ubiquitin
品牌介绍

泛素-蛋白酶体生物技术有限公司(UBPBio),LLC是一家私有公司,由科学家和投资人于2011年成立。UBPBio致力于为学术界和工业界提供高质量的试剂,以加快对泛素-蛋白酶体系统及相关途径的发现。 。目前,我们的产品线包括16个类别,提供350多种产品。如果您在泛素领域需要特殊试剂,请与我们联系,我们将竭诚为您服务。 

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