M-XStablePrestainedProteinLadderisathree-colorproteinstandardwith10pre-stainedproteinscoveringawiderangemolecularweightsfor10to180kDa.Proteinsarecovalentlycoupledwithabluechromophoreexceptfortworeferencebands(onegreenandoneredbandat25kDaand75kDa,respectively)whenseparatedonSDS-PAGE(Tris-glycinebuffer).TheM-XStablePrestainedProteinLadderisdesignedformonitoringproteinseparatedduringSDS-polyacrylamidegelelectrophoresis,verificationofWesterntransferefficiencyonmembranes(PVDF,nylon,ornitrocellulose)andforapproximatesizingofproteins.Theladderissuppliedingello
ADIngbufferandisreadytouse.Donotheat,dilute,addreducingagentbeforeloading.
ProductName: | M-XStablePrestainedProteinLadder |
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CatalogNo.: | L2010 |
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Size: | 500µl |
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Range: | ~10kDato~180kDa |
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NumberofBands: | 10 |
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Concentration: | Approximately0.2~0.4mg/mlofeachproteininbuffer(20mMTrisphosphatepH7.5at25°C),2%SDS,0.2mMDithiothreitol,3.6MUrea,and15%(v/v)glycerol). |
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RecommendedLoad: | •3μlor5μlperloadingforclearvisualizationduringelectrophoresison15-wellor10-wellmini-gel,respectively •2~3μlperwellforgeneralWesterntransfering. •Applymoreforthicker(>1.5mm)orlargergel. |
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StABIlity: | Stableforupto2weeksat25°C Stableforupto3monthsat4°C Forlongtermstorage,storeat-20°C(Recommended) |
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PDFDataSheet: | PDFDatasheet |
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Image(s): | |
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ShippingMethod: | Roomtemperatureshipping |
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Details
M-XStablePrestainedProteinLadderisathree-colorproteinstandardwith10pre-stainedproteinscoveringawiderangemolecularweightsfor10to180kDa.Proteinsarecovalentlycoupledwithabluechromophoreexceptfortworeferencebands(onegreenandoneredbandat25kDaand75kDa,respectively)whenseparatedonSDS-PAGE(Tris-glycinebuffer).TheM-XStablePrestainedProteinLadderisdesignedformonitoringproteinseparatedduringSDS-polyacrylamidegelelectrophoresis,verificationofWesterntransferefficiencyonmembranes(PVDF,nylon,ornitrocellulose)andforapproximatesizingofproteins.Theladderissuppliedingelloadingbufferandisreadytouse.Donotheat,dilute,addreducingagentbeforeloading.
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