Nickelchelateresinsrecognizetwoexposedhistidinesforanappreciableretentionofaproteinwitha4Xor6XHistag.NickelXPureAgroseResinprovidestheadvantagesoftheNickelcationwiththehighflowratesofthehighlycross-linkedagarosebeads.Exceptforbatchpurification,thisproductisspeciallysuitableforlargescaleHis-taggedproteinpurificationusingFPLC.TheXPureseriesofresinsaremadeforlargescaleandfinepurificationusingahigh-performanceliquidchromatography(HPLC)system.
Ube2IwaspurifiedwithNi-IDAXPureAgaroseResinusingagravitycolumn
Lane1: Molecularweight
MarkerLane2: Cellpelletaftersonication
Lane3: SupernatantaftersonicationLane4: Flowthroughafterbinding
Lane5:WashLane6: Elution1
Lane7:Elution2Lane8: Elution3
Lane9: Elution4Lane10: Elution5