产品编号：P1001-1000
市 场 价：¥6300.00
场 地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
邮 箱：
info@ebiomall.com
商品介绍
TobaccoEtchVirus(TEV)proteaseisacysteineproteasethatiscommonlyusedtoremovefusiontags,includingHis,GSTandMBP,attheNorCterminiofrecombinantproteins.TEVproteasespecificallyrecognizesasevenaminoacidsequenceGlu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser(ENLYFQG/S)andcleavesbetweenGlnandGly/Serunderawiderangeofconditions.
Recombinant6xHis-TEVwasexpressedinE.Coliandpurifiedtonearhomogeneity.ASer219Asn(S219N)substitutionwasintroducedthathasnoeffectonTEVproteaseactivity,butinhibitsauto-cleavageandthus,stABIlizingTEVproteaseduringincubationandlong-termstorage.
Werecommendusing3%suppliedTEVprotease(w/w)topreformreactionsat40Cfor12-16horat300Cfor1-4h.Dependingonspecificsubstrates,TEVproteaseconcentration,incubationtime,andreactiontemperatureshouldbeoptimized.6xHis-TEVcanberemovedbyNi-NTAresinafterincubation.
TEVproteasemaintainshighactivityunderawiderangeoftemperatures(40C-300C),pH(6.0-8.5)andbuffers,andinthepresenceofcommonlyusedproteaseinhibitors.
Recombinant6xHis-TEVwasexpressedinE.Coliandpurifiedtonearhomogeneity.ASer219Asn(S219N)substitutionwasintroducedthathasnoeffectonTEVproteaseactivity,butinhibitsauto-cleavageandthus,stABIlizingTEVproteaseduringincubationandlong-termstorage.
Werecommendusing3%suppliedTEVprotease(w/w)topreformreactionsat40Cfor12-16horat300Cfor1-4h.Dependingonspecificsubstrates,TEVproteaseconcentration,incubationtime,andreactiontemperatureshouldbeoptimized.6xHis-TEVcanberemovedbyNi-NTAresinafterincubation.
TEVproteasemaintainshighactivityunderawiderangeoftemperatures(40C-300C),pH(6.0-8.5)andbuffers,andinthepresenceofcommonlyusedproteaseinhibitors.
AdditionalInformation
| ProductName: | 6xHis-TEVProtease |
|---|---|
| AlsoKnownAs: | TEVprotease,NIa,Tobaccoetchvirusprotease |
| CatalogNo.: | P1001-1000 |
| Size: | 1mg |
| MolecularWeight: | 27kDa |
| Species: | TabaccoEtchVirus |
| Source: | Bacterialrecombinant |
| Stock: | 100mMTris,pH8.5at40C,500mMNaCl,5mMDTT,0.5mMEDTAand50%Glycerol |
| Concentration: | Seetubelabel |
| QualityAssurance: | >90%puritybasedonSDS-PAGE. |
| Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles |
| PDFDataSheet: | PDFDatasheet，MSDS |
| NCBIRefSeq: | N/A |
| Image(s): |  |
| ShippingMethod: | Dryiceshipping |
| References: | 1.LucastLJ.,etal.(2001)Biotechniques30,544. |
