Interferon–stimulatedgene15(Isg15)isa15kDaUb–likeprotein.ItislinkedtoproteinsinmuchthesamewayasUb,usingtheE1,E2,E3cascade.UbE1LandUbcH8arethespecificE1andE2,respectively,tomediateproteinISGylation.SeveralE3shavealsobeenfound.Isg15isinducedbytypeIinterferons(IFNα/β)andbacterialandviralinfections.Studiesarestillbeingconductedtodiscoverthe
BIOLOGicalfunctionsofIsg15.mISG15-GFPisafusionproteinwithmouseISG15fusedattheN-terminusofGFP.ItwasexpressedandpurifiedfromE.coli.
| ProductName: | 6xHis-mISG15-GFP |
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| AlsoKnownAs: | G1P2;IP17;UCRP;IFI15;hUCRP |
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| CatalogNo.: | E2200 |
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| Size: | 25µg |
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| MolecularWeight: | 44.9kDa |
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| Species: | Mouse |
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| Source: | Bacterialrecombinant |
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| Stock: | 20mMTris,150mMNaCl,2mMβME,10%Glycerol |
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| Concentration: | Seetubelabel |
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| QualityAssurance: | ~95%bySDS-PAGE,seedatasheetforgelimage |
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| Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles |
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| PDFDataSheet: | PDFDatasheet,MSDS |
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| NCBIRefSeq: | NM_005101 |
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| Image(s): | No |
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| ShippingMethod: | Dryiceshipping |
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| References: | 1.D’CunhaJ,etal.(1996)ProcNatlAcadSciUSA93(1),211–215. 2.D’CunhaJ,etal.(1996)JImmunol157(9),4100–4108.3.ZhangD,etal.(2011)JInterferonCytokineRes21(1),119–130.
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Details
Interferon–stimulatedgene15(Isg15)isa15kDaUb–likeprotein.ItislinkedtoproteinsinmuchthesamewayasUb,usingtheE1,E2,E3cascade.UbE1LandUbcH8arethespecificE1andE2,respectively,tomediateproteinISGylation.SeveralE3shavealsobeenfound.Isg15isinducedbytypeIinterferons(IFNα/β)andbacterialandviralinfections.StudiesarestillbeingconductedtodiscoverthebiologicalfunctionsofIsg15.mISG15-GFPisafusionproteinwithmouseISG15fusedattheN-terminusofGFP.ItwasexpressedandpurifiedfromE.coli.
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