ThiskitisdesignatedformonitoringCHIP-mediatedubiquitinationofuser-suppliedmisfoldedproteins.CHIPisanE3ubiquitinligasethatdirectlyorviaHsp70interactswithmisfoldedproteinsandpromotestheirpolyubiquitination,whichtargetsmisfoldedproteinsforproteasomaldegradation.
AdditionalInformation
| ProductName: | MisfoldedProteinUbiquitinationKit |
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| AlsoKnownAs: | N/A |
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| CatalogNo.: | J5110 |
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| Size: | 1Kit |
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| MolecularWeight: | N/A |
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| Species: | N/A |
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| Source: | N/A |
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| Stock: | Enzymestockedin20mMTris,150mMNaCl,2mMβME,10%Glycerol |
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| Concentration: | Seetubelabel |
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| QualityAssurance: | Enzyme>95%bySDS-PAGE |
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| Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles |
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| PDFDataSheet: | PDFdatasheet,MSDS |
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| NCBIRefSeq: | N/A |
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| Image(s): | No |
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| ShippingMethod: | Dryiceshipping |
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| References: | N/A |
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Details
*Thiskitisenoughforsettingup20x20µlreactions.
DescriptionThiskitisdesignatedformonitoringCHIP-mediatedubiquitinationofuser-suppliedmisfoldedproteins.CHIPisanE3ubiquitinligasethatdirectlyorviaHsp70interactswithmisfoldedproteinsandpromotestheirpolyubiquitination,whichtargetsmisfoldedproteinsforproteasomaldegradation.
Components
•10xHumanUbiquitin(500uM)..............................................40µl•20xUBE1(E1,2uM))............................................................20µl
•10x6xHis-UbE2D3(E2,20uM)............................................40µl•20x6XHis-CHIP(E3,40uM))................................................20µl
•10xHsp70(20uM)................................................................40µl•5xUbiquitinationBuffer.......................................................150µl
•10xATP(40mM)...................................................................60µl
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