Ni-NTAXPureAgaroseResincanbeusedtopurify6xHis-taggedproteinsexpressedinseriesofexpressionvectors,suchasE.coli.,yeast,insectcellsandmammaliancells.Ni-NTAXPureAgaroseResinconsistsof90µmbeadsofhighlycross-linked6%agarose,towhichNitilotriaceticacid(NTA)hasbeencoupled.Thechelatinggrouphasthenbeenchargedwithnickelions(Ni2+).Thisformisverystableoctahedralstructureofnickelionsinthecenter,whichcanprotectthenickelionsfromattackofthecompetitivesmallmolecule.ThestructureofNi-NTAiscompat
IBLewithacertainconcentrationofreducingagents,denaturingagents,detergentsandotheradditives.Ni-NTAXPureAgaroseResinishighlystableandexpandtherangeofsuitableoperatingconditions.
ProductName: | Ni-NTAXPureAgaroseResin |
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CatalogNo.: | P3020-5 |
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Size: | 5ml |
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Bead(Geometry,size): | 45-165µm |
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Cross-Linked: | Yes |
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Ligand: | Nitilotriaceticacid(NTA) |
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Agarose%: | Highlycross-linked6%agarose |
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BindingCapacity: | >40mg6xHis-taggedprotein/mlmedium |
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Volume%: | 50%(v/v)aqueoussUSPensioncontaining20%Ethanol |
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StorageTemperature: | 2-8°C |
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Application: | Batch,Gravity,MPLCandFPLC |
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PDFDataSheet: | PDFDatasheet |
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User"sManual: | PDFUser’sGuide |
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Image(s): | No |
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ShippingMethod: | Weticeshipping |
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Details
Ni-NTAXPureAgaroseResincanbeusedtopurify6xHis-taggedproteinsexpressedinseriesofexpressionvectors,suchasE.coli.,yeast,insectcellsandmammaliancells.Ni-NTAXPureAgaroseResinconsistsof90µmbeadsofhighlycross-linked6%agarose,towhichNitilotriaceticacid(NTA)hasbeencoupled.Thechelatinggrouphasthenbeenchargedwithnickelions(Ni2+).Thisformisverystableoctahedralstructureofnickelionsinthecenter,whichcanprotectthenickelionsfromattackofthecompetitivesmallmolecule.ThestructureofNi-NTAiscompatiblewithacertainconcentrationofreducingagents,denaturingagents,detergentsandotheradditives.Ni-NTAXPureAgaroseResinishighlystableandexpandtherangeofsuitableoperatingconditions.
TheXPureseriesofresinsaremadeforlargescaleandfinepurificationusingahigh-performaceliquidchromatography(HPLC)system.
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