| ProductName: | Anti-Ubiquitin(P4D1) |
|---|---|
| AlsoKnownAs: | Anti-Ub |
| CatalogNo.: | Y3011 |
| Size: | 100µg |
| MolecularWeight: | ~55/25kDa |
| Species: | Recognizesubiquitininawiderangeofspecies |
| Source: | Immunogen:Fulllengthbovineubiquitin |
| Stock: | lyophilizedpowder |
| Concentration: | lyophilizedpowder |
| QualityAssurance: | Guaranteedfordetecting5ngendogenousubiquitinorpolyubiquitinchainsinwholecellortissuelysatesatanantibodydilutionof1:1,000. |
| Storage: | 1)Storethelyophilizedantibodydesccatedat-20°C.2)Afterreconstitution,storetheantibodyat-20°C.Aliquotisnotnecessary.Thequalityoftheantibodyisguaranteedforoneyearafterpuchasingifstoredcorrectly. |
| PDFDataSheet: | PDFDatasheet |
| NCBIRefSeq: | N/A |
| Image(s): | (Clickimagetoenlarge)![]() Figure1.WesternblotapplicationsAnti-Ubiquitin(P4D1)wasusedata1:500dilution.Bovinethymusubiquitinwasrunasfollows;Lane1-50ng,Lane2-25ng.Lane3-12.5ng,Lane4-6.25ng,Lane5-3.12ng.Lanes6&7represent20μgofSwiss3T3celllysatefromcellstreatedfor5hwith10µMMG-132(Lane6)oruntreatedcells(Lane7).Arrowindicatesfreeubiquitinband(8kD),highermolecularweightbandsareubiquitinatedproteins. ![]() Figure2.IFofHeLacellsHeLacellswerestainedandvisualizedbyfluorescencemicroscopy.UbiquitintargetedcytoplasmicandnuclearproteinsandfreeubiquitinwerestainedusingAnti-Ubiquitin(P4D1)at1:500dilution. |
| ShippingMethod: | Roomtemperatureshipping |
| References: | N/A |