请使用支持JavaScript的浏览器!
主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ubpbio/Anti-Ubiquitin (P4D1)/Y3011/100 µg
产品编号:Y3011
市  场 价:¥3600.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$180.00
品      牌: UBPBio
公司分类:
ubpbio/Anti-Ubiquitin (P4D1)/Y3011/100 µg
商品介绍
Anti-UbMouseMonoclonalAntibody

AdditionalInformation

Details

Specificity
Recognizesmonoandpolyubiquitinatedproteins,freepolyubiquitinchainsandubiquitininawiderangeofspecies.Thisantibodyrecognizesdifferenttypesofpolyubiqutinchains.
IsotypeMouseIgG1
CloneP4D1
PurificationProteinGaffinitychromatography
ApplicationsWBandIF
WorkingConditionsWB(withECL):1:1000~1:3000dilution
IF:1:500IP:notrecommended
ForbestresultsofWBandIF,andotherassays(e.g.:DotandELISA),pleasedetermineoptimalworkingdilutionbytitrationtest
ReconstitutionCentrifugebrieflytocollectthepowdertothebottomofthetubepriortoreconstitution.Reconstituteeach100μgantibodyinto100μL50%glycerolinmiliQwater.Finalbuffercompositionis50mMPIPES,Ph7.0,1%sucrose,0.5%dextranand50%glycerol.Add0.02%azidetopreventbacterialcotamination.
Images(Clickimagetoenlarge)

Figure1.WesternblotapplicationsAnti-Ubiquitin(P4D1)wasusedata1:500dilution.Bovinethymusubiquitinwasrunasfollows;Lane1-50ng,Lane2-25ng.Lane3-12.5ng,Lane4-6.25ng,Lane5-3.12ng.Lanes6&7represent20μgofSwiss3T3celllysatefromcellstreatedfor5hwith10µMMG-132(Lane6)oruntreatedcells(Lane7).Arrowindicatesfreeubiquitinband(8kD),highermolecularweightbandsareubiquitinatedproteins.

Figure2.IFofHeLacellsHeLacellswerestainedandvisualizedbyfluorescencemicroscopy.UbiquitintargetedcytoplasmicandnuclearproteinsandfreeubiquitinwerestainedusingAnti-Ubiquitin(P4D1)at1:500dilution.
品牌介绍

泛素-蛋白酶体生物技术有限公司(UBPBio),LLC是一家私有公司,由科学家和投资人于2011年成立。UBPBio致力于为学术界和工业界提供高质量的试剂,以加快对泛素-蛋白酶体系统及相关途径的发现。 。目前,我们的产品线包括16个类别,提供350多种产品。如果您在泛素领域需要特殊试剂,请与我们联系,我们将竭诚为您服务。 

自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
ProductName:Anti-Ubiquitin(P4D1)
AlsoKnownAs:Anti-Ub
CatalogNo.:Y3011
Size:100µg
MolecularWeight:~55/25kDa
Species:Recognizesubiquitininawiderangeofspecies
Source:Immunogen:Fulllengthbovineubiquitin
Stock:lyophilizedpowder
Concentration:lyophilizedpowder
QualityAssurance:Guaranteedfordetecting5ngendogenousubiquitinorpolyubiquitinchainsinwholecellortissuelysatesatanantibodydilutionof1:1,000.
Storage:1)Storethelyophilizedantibodydesccatedat-20°C.2)Afterreconstitution,storetheantibodyat-20°C.Aliquotisnotnecessary.Thequalityoftheantibodyisguaranteedforoneyearafterpuchasingifstoredcorrectly.
PDFDataSheet:PDFDatasheet
NCBIRefSeq:N/A
Image(s):(Clickimagetoenlarge)

Figure1.WesternblotapplicationsAnti-Ubiquitin(P4D1)wasusedata1:500dilution.Bovinethymusubiquitinwasrunasfollows;Lane1-50ng,Lane2-25ng.Lane3-12.5ng,Lane4-6.25ng,Lane5-3.12ng.Lanes6&7represent20μgofSwiss3T3celllysatefromcellstreatedfor5hwith10µMMG-132(Lane6)oruntreatedcells(Lane7).Arrowindicatesfreeubiquitinband(8kD),highermolecularweightbandsareubiquitinatedproteins.

Figure2.IFofHeLacellsHeLacellswerestainedandvisualizedbyfluorescencemicroscopy.UbiquitintargetedcytoplasmicandnuclearproteinsandfreeubiquitinwerestainedusingAnti-Ubiquitin(P4D1)at1:500dilution.
ShippingMethod:Roomtemperatureshipping
References:N/A