ProductName: | Anti-Ubiquitin(P4D1) |
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AlsoKnownAs: | Anti-Ub |
CatalogNo.: | Y3011 |
Size: | 100µg |
MolecularWeight: | ~55/25kDa |
Species: | Recognizesubiquitininawiderangeofspecies |
Source: | Immunogen:Fulllengthbovineubiquitin |
Stock: | lyophilizedpowder |
Concentration: | lyophilizedpowder |
QualityAssurance: | Guaranteedfordetecting5ngendogenousubiquitinorpolyubiquitinchainsinwholecellortissuelysatesatanantibodydilutionof1:1,000. |
Storage: | 1)Storethelyophilizedantibodydesccatedat-20°C.2)Afterreconstitution,storetheantibodyat-20°C.Aliquotisnotnecessary.Thequalityoftheantibodyisguaranteedforoneyearafterpuchasingifstoredcorrectly. |
PDFDataSheet: | PDFDatasheet |
NCBIRefSeq: | N/A |
Image(s): | (Clickimagetoenlarge) Figure1.WesternblotapplicationsAnti-Ubiquitin(P4D1)wasusedata1:500dilution.Bovinethymusubiquitinwasrunasfollows;Lane1-50ng,Lane2-25ng.Lane3-12.5ng,Lane4-6.25ng,Lane5-3.12ng.Lanes6&7represent20μgofSwiss3T3celllysatefromcellstreatedfor5hwith10µMMG-132(Lane6)oruntreatedcells(Lane7).Arrowindicatesfreeubiquitinband(8kD),highermolecularweightbandsareubiquitinatedproteins. Figure2.IFofHeLacellsHeLacellswerestainedandvisualizedbyfluorescencemicroscopy.UbiquitintargetedcytoplasmicandnuclearproteinsandfreeubiquitinwerestainedusingAnti-Ubiquitin(P4D1)at1:500dilution. |
ShippingMethod: | Roomtemperatureshipping |
References: | N/A |