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℡ 4000-520-616
℡ 4000-520-616
ubpbio/Biotin-SUMO2-VME/M2010/25 µg
产品编号:M2010
市  场 价:¥5000.00
场      地:美国(厂家直采)
产品分类: 蛋白类>>蛋白酶类>
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美  元  价:$250.00
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ubpbio/Biotin-SUMO2-VME/M2010/25 µg
商品介绍
Apotent,irreversIBLeandspecificinhibitorofdeSUMOylationproteases(SENPs)thatcleaveSUMO2conjugates.ItisN-terminallytaggedwithabiotin,whichallowsforsensitiveidentificationofSENPsusingHRP-conjugatedstreptavidininimmunoblottingassays,orpurificationofSENPsusingstreptavidinresin.ThebiotintagisseparatedfromSUMO2byanaminohexanoicacid(Ahx)linkerthatcouldincreasethereactivityofthebiotinmoiety.Inaddition,Cys48inSUMO2wasmutatedtoSer.

AdditionalInformation

Details

Apotent,irreversibleandspecificinhibitorofdeSUMOylationproteases(SENPs)thatcleaveSUMO2conjugates.ItisN-terminallytaggedwithabiotin,whichallowsforsensitiveidentificationofSENPsusingHRP-conjugatedstreptavidininimmunoblottingassays,orpurificationofSENPsusingstreptavidinresin.ThebiotintagisseparatedfromSUMO2byanaminohexanoicacid(Ahx)linkerthatcouldincreasethereactivityofthebiotinmoiety.Inaddition,Cys48inSUMO2wasmutatedtoSer.

SamplePreparation:
Briefcentrifugationat10,000xgfor2minutestopelletthepowder.DissolvethepowderinsmallamountofDMSO(e.g.25μgin0.625μL=40μg/μL).AddthisDMSOstockslowlyto50μLbuffersuchas20mMTris,pH7.2,50mMNaCltomakeastocksolutionof0.5μg/μL(45μM)(pleasenotetheorderofaddition).Tapthetubetomix.Workingconcentration0.5-2μM.
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ProductName:Biotin-SUMO2-VME
AlsoKnownAs:N/A
CatalogNo.:M2010
Size:25µg
MolecularWeight:11.02kDa
Species:Human
Source:Synthetic
Stock:Productinlyophilizedpowderform.
Concentration:N/A
QualityAssurance:≥95%byRP-HPLC
Storage:Powderat−20°C;solutionat−80°C.Avoidmultiplefreeze/thawcycles.
PDFDataSheet:PDFDatasheetMSDS
NCBIRefSeq:N/A
Image(s):No
ShippingMethod:Roomtemperatureshipping
References:1.Albrowetal.,(2011)ChemBiol18,722.
2.Mendesetal.,(2016)BiochimBiophysActa-MolCellRes.1863,139.