Apotent,irrevers
IBLeandspecificinhibitorofdeSUMOylationproteases(SENPs)thatcleaveSUMO2conjugates.ItisN-terminallytaggedwithabiotin,whichallowsforsensitiveidentificationofSENPsusingHRP-conjugatedstreptavidininimmunoblottingassays,orpurificationofSENPsusingstreptavidinresin.ThebiotintagisseparatedfromSUMO2byanaminohexanoicacid(Ahx)linkerthatcouldincreasethereactivityofthebiotinmoiety.Inaddition,Cys48inSUMO2wasmutatedtoSer.
AdditionalInformation
| ProductName: | Biotin-SUMO2-VME |
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| AlsoKnownAs: | N/A |
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| CatalogNo.: | M2010 |
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| Size: | 25µg |
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| MolecularWeight: | 11.02kDa |
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| Species: | Human |
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| Source: | Synthetic |
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| Stock: | Productinlyophilizedpowderform. |
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| Concentration: | N/A |
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| QualityAssurance: | ≥95%byRP-HPLC |
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| Storage: | Powderat−20°C;solutionat−80°C.Avoidmultiplefreeze/thawcycles. |
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| PDFDataSheet: | PDFDatasheet,MSDS |
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| NCBIRefSeq: | N/A |
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| Image(s): | No |
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| ShippingMethod: | Roomtemperatureshipping |
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| References: | 1.Albrowetal.,(2011)ChemBiol18,722. 2.Mendesetal.,(2016)BiochimBiophysActa-MolCellRes.1863,139. |
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Details
Apotent,irreversibleandspecificinhibitorofdeSUMOylationproteases(SENPs)thatcleaveSUMO2conjugates.ItisN-terminallytaggedwithabiotin,whichallowsforsensitiveidentificationofSENPsusingHRP-conjugatedstreptavidininimmunoblottingassays,orpurificationofSENPsusingstreptavidinresin.ThebiotintagisseparatedfromSUMO2byanaminohexanoicacid(Ahx)linkerthatcouldincreasethereactivityofthebiotinmoiety.Inaddition,Cys48inSUMO2wasmutatedtoSer.
SamplePreparation:
Briefcentrifugationat10,000xgfor2minutestopelletthepowder.DissolvethepowderinsmallamountofDMSO(e.g.25μgin0.625μL=40μg/μL).AddthisDMSOstockslowlyto50μLbuffersuchas20mMTris,pH7.2,50mMNaCltomakeastocksolutionof0.5μg/μL(45μM)(pleasenotetheorderofaddition).Tapthetubetomix.Workingconcentration0.5-2μM.
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