Cy5-Ub-VMEisapotent,irrevers
IBLeandspecificinhibitorofdeubiquitinatingenzymes(DUBs),whichislabeledontheN-terminuswithaCy5dye(Cy5,Ex625-650nm,Em670nm).Thisubiquitin-basedactivityprobecanbeusedforactivityprofilingexperimentsandlabelingofDUBswithafluorescentdye(Cy5).Cy5labellingallowsfordetectionofDUBlabelingbyin-gelfluorescence.
| ProductName: | Cy5-Ub-VME |
|---|
| AlsoKnownAs: | N/A |
|---|
| CatalogNo.: | M1040 |
|---|
| Size: | 25µg |
|---|
| MolecularWeight: | 9.1kDa |
|---|
| Species: | Human |
|---|
| Source: | Synthetic |
|---|
| Stock: | Productinlyophilizedpowderform. |
|---|
| Concentration: | N/A |
|---|
| QualityAssurance: | ≥95%byRP-HPLC |
|---|
| Storage: | Powderat−20°C;solutionat−80°C.Avoidmultiplefreeze/thawcycles. |
|---|
| PDFDataSheet: | PDFDatasheet,MSDS |
|---|
| NCBIRefSeq: | N/A |
|---|
| Image(s): | No |
|---|
| ShippingMethod: | Roomtemperatureshipping |
|---|
| References: | 1.Altunetal.,(2011)ChemBiol18,1401. 2.Misaghietal.,(2005)J.Biol.Chem.280,1512. |
|---|
Details
Cy5-Ub-VMEisapotent,irreversibleandspecificinhibitorofdeubiquitinatingenzymes(DUBs),whichislabeledontheN-terminuswithaCy5dye(Cy5,Ex625-650nm,Em670nm).Thisubiquitin-basedactivityprobecanbeusedforactivityprofilingexperimentsandlabelingofDUBswithafluorescentdye(Cy5).Cy5labellingallowsfordetectionofDUBlabelingbyin-gelfluorescence.
SamplePreparation:
DissolvethepowderinDMSO(e.g.add1.25μlDMSOto25μgpowdertoget20mg/mlor2.25mMDMSOstock)andaddthisDMSOstocktotherequiredbuffer(pleasenotetheorderofaddition).Typicalreactionconcentrationsvaryfrom0.2-2μM.
泛素-蛋白酶体生物技术有限公司(UBPBio),LLC是一家私有公司,由科学家和投资人于2011年成立。UBPBio致力于为学术界和工业界提供高质量的试剂,以加快对泛素-蛋白酶体系统及相关途径的发现。 。目前,我们的产品线包括16个类别,提供350多种产品。如果您在泛素领域需要特殊试剂,请与我们联系,我们将竭诚为您服务。