Cy5-Ub-VMEisapotent,irrevers
IBLeandspecificinhibitorofdeubiquitinatingenzymes(DUBs),whichislabeledontheN-terminuswithaCy5dye(Cy5,Ex625-650nm,Em670nm).Thisubiquitin-basedactivityprobecanbeusedforactivityprofilingexperimentsandlabelingofDUBswithafluorescentdye(Cy5).Cy5labellingallowsfordetectionofDUBlabelingbyin-gelfluorescence.
ProductName: | Cy5-Ub-VME |
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AlsoKnownAs: | N/A |
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CatalogNo.: | M1040 |
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Size: | 25µg |
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MolecularWeight: | 9.1kDa |
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Species: | Human |
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Source: | Synthetic |
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Stock: | Productinlyophilizedpowderform. |
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Concentration: | N/A |
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QualityAssurance: | ≥95%byRP-HPLC |
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Storage: | Powderat−20°C;solutionat−80°C.Avoidmultiplefreeze/thawcycles. |
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PDFDataSheet: | PDFDatasheet,MSDS |
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NCBIRefSeq: | N/A |
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Image(s): | No |
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ShippingMethod: | Roomtemperatureshipping |
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References: | 1.Altunetal.,(2011)ChemBiol18,1401. 2.Misaghietal.,(2005)J.Biol.Chem.280,1512. |
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Details
Cy5-Ub-VMEisapotent,irreversibleandspecificinhibitorofdeubiquitinatingenzymes(DUBs),whichislabeledontheN-terminuswithaCy5dye(Cy5,Ex625-650nm,Em670nm).Thisubiquitin-basedactivityprobecanbeusedforactivityprofilingexperimentsandlabelingofDUBswithafluorescentdye(Cy5).Cy5labellingallowsfordetectionofDUBlabelingbyin-gelfluorescence.
SamplePreparation:
DissolvethepowderinDMSO(e.g.add1.25μlDMSOto25μgpowdertoget20mg/mlor2.25mMDMSOstock)andaddthisDMSOstocktotherequiredbuffer(pleasenotetheorderofaddition).Typicalreactionconcentrationsvaryfrom0.2-2μM.
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