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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ubpbio/Biotin-Ub-PA/M1020/25 µg
产品编号:M1020
市  场 价:¥4300.00
场      地:美国(厂家直采)
产品分类: 蛋白类>>蛋白酶类>
联系QQ:1570468124
电话号码:4000-520-616
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美  元  价:$215.00
品      牌: UBPBio
公司分类:
ubpbio/Biotin-Ub-PA/M1020/25 µg
商品介绍
Biotin-Ub-PAisanewlydevelopedpotentandspecificinhibitorofdeubiquitinatingenzymes(DUBs),whichhasaN-terminalbiotintag.Anaminohexanoicacid(Ahx)linkerisusedtocreateextraspacebetweenthebiotinandUbproteinforefficientaccessofthebiotinbindingentity.ThisactivityprobecanbeusedforactivityprofilingexperimentsanddeterminingDUBinhibitorspecificity.IthastwouniquecapABIlities:itformsacovalentlinkagewiththeactivesiteCysresidueofaDUBthatcanbecleavedbyacidtreatment(5%aq.TFA),allowingforproteomicanalyses.Biotin-Ub-PAtargetsallthreemajorDUBfamilies:UCH,USPandOTU

AdditionalInformation

Details

Biotin-Ub-PAisanewlydevelopedpotentandspecificinhibitorofdeubiquitinatingenzymes(DUBs),whichhasaN-terminalbiotintag.Anaminohexanoicacid(Ahx)linkerisusedtocreateextraspacebetweenthebiotinandUbproteinforefficientaccessofthebiotinbindingentity.ThisactivityprobecanbeusedforactivityprofilingexperimentsanddeterminingDUBinhibitorspecificity.Ithastwouniquecapabilities:itformsacovalentlinkagewiththeactivesiteCysresidueofaDUBthatcanbecleavedbyacidtreatment(5%aq.TFA),allowingforproteomicanalyses.Biotin-Ub-PAtargetsallthreemajorDUBfamilies:UCH,USPandOTU

SamplePreparation:
DissolvethepowderinDMSO(e.g.add1.25μlDMSOto25μgpowdertoget20mg/mlor2.25mMDMSOstock)andaddthisDMSOstockslowlytotherequiredbuffer(pleasenotetheorderofaddition).Typicreactionconcentrationsvaryfrom0.2-2mM.Images:(Clickimagetoenlarge)
ProductName:Biotin-Ub-PA
AlsoKnownAs:N/A
CatalogNo.:M1020
Size:25µg
MolecularWeight:8885DabyMS
Species:Human
Source:Synthetic
Stock:Productinlyophilizedpowderform.
Concentration:N/A
QualityAssurance:≥95%byRP-HPLCandSDS-PAGE
Storage:Powderat−20°C;solutionat−80°C.Avoidmultiplefreeze/thawcycles.
PDFDataSheet:PDFDatasheetMSDS
NCBIRefSeq:N/A
Image(s):(Clickimagetoenlarge)
SDS-PAGEanalysis.
Coommassiebluestaining,12%SDS-PAGEgel.
ShippingMethod:Roomtemperatureshipping
References:1.Ekkebusetal.,(2013)J.Am.Chem.Soc.135,2867.
2.Sommeretal.,(2013)Bioorg.Med.Chem.21,2511.3.Galardyetal.,(2005)MethodsinEnzymology399,120.
4.deJongetal.,(2012)ChemBioChem13,2251.
SDS-PAGEanalysis.
Coommassiebluestaining,12%SDS-PAGEgel.
品牌介绍

泛素-蛋白酶体生物技术有限公司(UBPBio),LLC是一家私有公司,由科学家和投资人于2011年成立。UBPBio致力于为学术界和工业界提供高质量的试剂,以加快对泛素-蛋白酶体系统及相关途径的发现。 。目前,我们的产品线包括16个类别,提供350多种产品。如果您在泛素领域需要特殊试剂,请与我们联系,我们将竭诚为您服务。 

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