ubpbio/Polyubiquitin Chain Capture Kit/J4460/1 Kit,UBPBio,,UBPBio,Ubiquitin-Proteasome Biotechnologies,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ubpbio/Polyubiquitin Chain Capture Kit/J4460/1 Kit

产品编号：J4460

市场价：¥7600.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>其它重组蛋白>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$380.00

品牌： UBPBio

公司：Ubiquitin-Proteasome Biotechnologies, LLC

公司分类：

商品介绍

ThiskitisdesignedforenrichmentofpolyubiquitinatedcellularproteinsusingGSTtaggedrecombinantproteinsthatbindlysine48andlysine63polyubiquitinchains.Itcanbeusedtoenrichpolyubiquitinatedproteinsinwholecellortissuelysates.Boundproteinscanbeprecipitatedusingglutathioneresinandelutedbyabuffercontaining10mMglutathione,andthenassayedbyim-munoblottingormassspectrometry.

AdditionalInformation

Details

Description
ThiskitisdesignedforenrichmentofpolyubiquitinatedcellularproteinsusingGSTtaggedrecombinantproteinsthatbindlysine48andlysine63polyubiquitinchains.Itcanbeusedtoenrichpolyubiquitinatedproteinsinwholecellortissuelysates.Boundproteinscanbeprecipitatedusingglutathioneresinandelutedbyabuffercontaining10mMglutathione,andthenassayedbyim-munoblottingormassspectrometry.
Components
•GST-S5a(UIM)/GST-TAB2(NZF)Mix(2mg/mleach)..............1ml
•GST(4mg/ml)...........................................................................0.5ml•Iodoacetamine(IAA)..................................................................25mg
•Glutathione(reduced)..............................................................100mg•GlutathioneAgaroseResin(75%slurry)..................................1.0ml
Images:
(Clickimagetoenlarge)

ProductName:

PolyubiquitinChainCaptureKit

AlsoKnownAs:

N/A

CatalogNo.:

J4460

Size:

1Kit

MolecularWeight:

N/A

Species:

N/A

Source:

N/A

Stock:

Enzymestockedin20mMTris,150mMNaCl,2mMβME,10%Glycerol

Concentration:

Seetubelabel

QualityAssurance:

Enzyme>95%bySDS-PAGE

Storage:

Storeat-80°C;avoidmultiplefreeze-thawcycles

PDFDataSheet:

PDFdatasheet,MSDS

NCBIRefSeq:

N/A

Image(s):

(Clickimagetoenlarge)

SchematicofpolyUbchainsenrichment

Lane1:GST
Lane2:GST-S5a(UIM)Lane3:GST-TAB2(NZF)
Lane4:GST-S5a(UIM)+GST-TAB2(NZF)Lane5:GST-NEMO(UBAN)
Accordingtoproceduresdescribedabove,5mgwholecelllysatesfromHeLacellsweremixedwith0.2mgGST(lane1),GST-S5a(UIM)(lane2),GST-TAB2(NZF)(lane3),GST-S5a(UIM)+GST-TAB2(NZF)(lane4),orGST-NEMO(UBAN)(lane5).10%eluateswereseparatedbySDS-PAGE,followedbyimmunoblottingofUb,K48-specificUbchainsandGST.
*NoimmunoreactivitywasdetectedinGST-NEMO(UBAN)pulldownassays,presumablybecausethereislittlelinearpolyubiquitinchainsincells.

ShippingMethod:

Dryiceshipping

References:

N/A

SchematicofpolyUbchainsenrichment

品牌介绍

泛素-蛋白酶体生物技术有限公司（UBPBio），LLC是一家私有公司，由科学家和投资人于2011年成立。UBPBio致力于为学术界和工业界提供高质量的试剂，以加快对泛素-蛋白酶体系统及相关途径的发现。。目前，我们的产品线包括16个类别，提供350多种产品。如果您在泛素领域需要特殊试剂，请与我们联系，我们将竭诚为您服务。

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