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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ubpbio/Unconjugated (Free) Polyubiquitin Chain Capture Kit/J4420/1 Kit
产品编号:J4420
市  场 价:¥7600.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$380.00
品      牌: UBPBio
公司分类:
ubpbio/Unconjugated (Free) Polyubiquitin Chain Capture Kit/J4420/1 Kit
商品介绍
TheUnconjugated(Free)PolyubiquitinChainCaptureKityenrichfreepolyUbchainsincellortissueextractswithin6hours.ThisapproachusestheN-terminalZnF-UBPdomainofthedeubiquitinatingenzymeUSP5tospecificallycapturefreepolyUbchains.TheZnF-UBPdomainofUsp5bindstheC-terminaldiglycinemotifinfreeUbchains,itdoesnotbindpolyUbchainsconjugatedonsubstrateproteins.

AdditionalInformation

Details

Thiskitissufficientforpurificationoffreepolyubiquitinchainsin100mgor20X5mgcellortissueextractsataconcentrationof5mg/ml.
Description
TheUnconjugated(Free)PolyubiquitinChainCaptureKityenrichfreepolyUbchainsincellortissueextractswithin6hours.ThisapproachusestheN-terminalZnF-UBPdomainofthedeubiquitinatingenzymeUsp5tospecificallycapturefreepolyUbchains.TheZnF-UBPdomainofUsp5bindstheC-terminaldiglycinemotifinfreeUbchains,itdoesnotbindpolyUbchainsconjugatedonsubstrateproteins.
Components
•GST-ZnF-UBP(4mg/ml)..............................................0.5ml•GST(4mg/ml).............................................................0.5ml
•Iodoacetamine(IAA)....................................................25mg•Glutathione(reduced).................................................100mg
•GlutathioneAgaroseResin(75%slurry).....................1.0ml
Images(Clickimagetoenlarge)

10µlGST-boundorGST-ZnF-UBP-boundproteinswereseparatedonSDS-PAGEsandimmunoblottedwithantibodiesagainUborK48-linkedpolyUbchains.
品牌介绍

泛素-蛋白酶体生物技术有限公司(UBPBio),LLC是一家私有公司,由科学家和投资人于2011年成立。UBPBio致力于为学术界和工业界提供高质量的试剂,以加快对泛素-蛋白酶体系统及相关途径的发现。 。目前,我们的产品线包括16个类别,提供350多种产品。如果您在泛素领域需要特殊试剂,请与我们联系,我们将竭诚为您服务。 

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ProductName:Unconjugated(Free)PolyubiquitinChainCaptureKit
AlsoKnownAs:N/A
CatalogNo.:J4420
Size:1Kit
MolecularWeight:No
Species:No
Source:No
Stock:Enzymestockedin20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:Enzyme>95%bySDS-PAGE
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFdatasheet,MSDS
NCBIRefSeq:N/A
Image(s):No
ShippingMethod:Dryiceshipping
References:No