Thiskitissufficientforpurificationofpolysumoylatedproteinsfrom100mgor20X5mgcellortissueextractsataconcentrationof5mg/ml.
DescriptionThePolySUMOCapture&ReleaseKitisdesignedtorapidlyenrichpolysumoylatedproteinsfromcellortissueextractswithin6hoursaccordingtoapublishedmethod(reference1)withmodifications.ThisapproachusestheSIMdomainsofRNF4tocapturepolysumoylatedproteins,whicharesubsequentreleasedbyincubatingwithacompetitivepeptidethatbindsSIM.
Components•GST-4XSIM(4mg/ml)..............................................1ml
•GST(4mg/ml).......................................................1.2ml
•2XElutionpeptide............................................0.45ml
•GlutathioneAgaroseResin(75%slurry).........1.0ml
•Iodoacetamine(IAA)............................................25mg
•20XCellLysisBuffer.....................................2X1.0ml
•1MNaCl.......................................................4X1.25ml
Images(Clickimagetoenlarge)

1.Celllysatesbeforepulldown
2.Celllysatesafterpulldown
3.Elution