TRABID is an OUT domain-containing deubiquitinating enzyme that exhibits specificity to K29/K33-linked polyubiquitin chains. 6xHis-GST-TRABID(NZF1) contains the N-terminal NZF1 domain (amino acid 1-33) of TRABID fused at the C-terminal of the 6xHis-GST tag. TRABID(NZF1) specifically binds K29/K33 polyubiquitin chains. This protein can be used to pull down K29 and K33 polyubiquitin chains using Ni-NTA resin or glutathione resin.
Additional Information
| Product Name: |
6xHis-GST-TRABID(NZF1) |
| Also Known As: |
NZF1 |
| Catalog No.: |
I1811 |
| Size: |
500 µg |
| Molecular Weight: |
29 kDa (with tags) |
| Species: |
Human |
| Source: |
Bacterial recombinant |
| Stock: |
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: |
See tube label |
| Quality Assurance: |
~85% by SDS-PAGE, see datasheet for gel image |
| Storage: |
Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: |
PDF Datasheet |
| NCBI RefSeq: |
N/A |
| Image(s): |
(Click image to enlarge)
|
Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-GST-TRABID(NZF1)
|
|
| Shipping Method: |
Dry ice shipping |
| References: |
Michel MA, et al. (2015) Mol Cell. 58(1):95-109
|
Details
TRABID is an OUT domain-containing deubiquitinating enzyme that exhibits specificity to K29/K33-linked polyubiquitin chains. 6xHis-GST-TRABID(NZF1) contains the N-terminal NZF1 domain (amino acid 1-33) of TRABID fused at the C-terminal of the 6xHis-GST tag. TRABID(NZF1) specifically binds K29/K33 polyubiquitin chains. This protein can be used to pull down K29 and K33 polyubiquitin chains using Ni-NTA resin or glutathione resin.
Images:
(Click image to enlarge)
|
Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-GST-TRABID(NZF1)
|