商品介绍
(Suc-LLVY)2-Rhodamineisafluorogenicsubstrateforthechymotrypsin–likeactivityofthe20Sand26Sproteasomes.Workingconcentrationsofthissubstrateis20-100µM.ThereleasedRhodamine110fluorescencecanbedetectedbyafluorimeterorplatereaderatexcitation/emissionof495nm/520nm,respectively.ComparedtoAMC,Rhodamine110isexcitedatlongerwavelengththatcansignificantlyenhancethesignal/noiseratio.ThissubstrateisextremlyvaluableforscreeningsmallchemicalinhibitorsofproteasomesbyavoidingtheinterferencefromchemicalcompoundautofluorescencethatoftenoccursatshorterwavelengthsaroundUVornear-UVregions.
Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.ReADIngsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.
Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.ReADIngsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.
AdditionalInformation
| ProductName: | (Suc-Leu-Leu-Val-Tyr)2-Rhodamine110 |
|---|---|
| AlsoKnownAs: | (Suc-LLVY)2-Rhodamine110 |
| CatalogNo.: | G1111 |
| Size: | 10mg |
| MolecularWeight: | 1507.7Da |
| Species: | N/A |
| Source: | Synthetic |
| Stock: | Powder |
| Concentration: | N/A |
| QualityAssurance: | >98%byHPLCandNMR |
| Storage: | EligIBLeforroomtemperatureshipping.Storeat-80°Cuponreceiving;avoidmultiplefreeze-thawcyclesafterdissolvinginDMSO. |
| PDFDataSheet: | DownloadPDFdatasheet,MSDS |
| NCBIRefSeq: | N/A |
| Image(s): | No |
| ShippingMethod: | Roomtemperatureshipping |
| References: | No |
