TheE2ScreeningKitisdevelopedforidentificationoftheE2Ub-conjugatingenzyme(s)foraspecificE3Ubligaseand/oraproteinsubstrate.
AdditionalInformation
ProductName: | E2ScreeningKit |
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AlsoKnownAs: | N/A |
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CatalogNo.: | J1100 |
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Size: | 1Kit |
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MolecularWeight: | N/A |
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Species: | Human |
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Source: | Bacterialrecombinant |
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Stock: | 20mMTris,150mMNaCl,2mMβME,10%Glycerol |
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Concentration: | Seetubelabel |
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QualityAssurance: | 90%-95%basedonSDS-PAGE |
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Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles |
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PDFDataSheet: | PDFdatasheet,MSDS |
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NCBIRefSeq: | N/A |
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Image(s): | No |
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ShippingMethod: | Dryiceshipping |
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References: | N/A |
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Details
Description
TheE2ScreeningKitisdevelopedforidentificationoftheE2Ub-conjugatingenzyme(s)foraspecificE3Ubligaseand/oraproteinsubstrate.
Components
•20XUBE1(2µM)......................................................100µl•10XE2Ubconjugatingenzymes
(29E2s,seetablebelow,20µM)................................20µleach•10XHumanUbiquitin(500µM)................................200µl
•10XUbiquitinationBuffer.............................................1ml•40mMATP...............................................................250µl
E2Name
1:6XHis-Ube2A(Rad6A)2:6XHis-Ube2B
3:6XHis-Ube2C(UbcH10)4:6XHis-Ube2D1(UbcH5A)
5:6XHis-Ube2D2(UbcH5B)6:6XHis-Ube2D3(UbcH5C)
7:6XHis-Ube2D4(UbcH5D)8:6XHis-Ube2E1(UbcH6)
9:6XHis-Ube2E2(UbcH8)10:6XHis-Ube2E3(UbcH9)
11:6XHis-Ube2F12:6XHis-Ube2G1
13:6XHis-Ube2G214:6XHis-Ube2H(UbcH2)
15:6XHis-Ube2I16:6XHis-Ube2J1(1-282)
17:GST-Ube2K(E2-25K)18:6XHis-Ube2L3
19:6XHis-Ube2L620:6XHis-Ube2M(UbcH12)
21:6XHis-UbeN(Ubc13/Uev1a)22:6XHis-Ube2Q2
23:6XHis-Ube2R1(Cdc34)24:6XHis-Ube2R2
25:6XHis-Ube2S26:6XHis-Ube2T
27:6xHis-Ubc13/UbE2V228:6XHis-Ube2W
29:6XHis-Ube2Z
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