Thiskitisdesignedforassaying26Sproteasome-mediateddegradationofubiquitinatedproteinsinvitro.Itcontainshighlypurifiedactivebovine26Sproteasomethathasbeenusedbydifferentresearchgroupsforassayingproteindegradation.
| ProductName: | 26SProteasome-mediatedProteinDegradationKit |
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| AlsoKnownAs: | N/A |
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| CatalogNo.: | J4330 |
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| Size: | 1Kit |
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| MolecularWeight: | N/A |
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| Species: | N/A |
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| Source: | N/A |
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| Stock: | Enzymestockedin20mMTris,150mMNaCl,2mMβME,10%Glycerol |
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| Concentration: | Seetubelabel |
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| QualityAssurance: | Enzyme>95%bySDS-PAGE |
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| Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles |
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| PDFDataSheet: | PDFdatasheet |
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| NCBIRefSeq: | N/A |
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| Image(s): | N/A |
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| ShippingMethod: | Dryiceshipping |
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| References: | 1.LiuCW,LiX,ThompsonD,WoodingK,ChangTL,TangZ,YuH,ThomasPJ,DeMartinoGN.MolCell.2006;24(1):39-50.
2.JacobsonAD,ZhangNY,XuP,HanKJ,NooneS,PengJ,LiuCW.JBiolChem.2009;284(51):35485-94.3.HemanthaHP,BavikarSN,Herman-BachinskyY,Haj-YahyaN,BondalapatiS,CiechanoverA,BrikA.JAmChemSoc.2014;136(6):2665-73.
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Details
*Thesuppliedmaterialsaresufficientforsettingup32X25ulreactions.
Description
Thiskitisdesignedforassaying26Sproteasome-mediateddegradationofubiquitinatedproteinsinvitro.Itcontainshighlypurifiedactivebovine26Sproteasomethathasbeenusedbydifferentresearchgroupsforassayingproteindegradation(seereferences).
Components
•Bovine26Sproteasome(200nM).............................100μl
•Epoxomicin(10mMinDMSO).....................................3μl•ATP(500mM).............................................................20μl
•10XDegradationBuffer.............................................150μl
Notes
1.10XDegradationBuffer:400mMTris,pH7.1at37°C,400mMNaCl,50mMMgCl2,20mMβ-ME.2.Thesuppliedproteasome-specificinhibitorepoxomicinwasdissolvedinDMSOat10mM,dilute100Xforuse.
References
1.LiuCW,LiX,ThompsonD,WoodingK,ChangTL,TangZ,YuH,ThomasPJ,DeMartinoGN.MolCell.2006;24(1):39-50.2.JacobsonAD,ZhangNY,XuP,HanKJ,NooneS,PengJ,LiuCW.JBiolChem.2009;284(51):35485-94.
3.HemanthaHP,BavikarSN,Herman-BachinskyY,Haj-YahyaN,BondalapatiS,CiechanoverA,BrikA.JAmChemSoc.2014;136(6):2665-73.
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