ProductName: | Bovineimmuno20Sproteasome | |||||||||||||||
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AlsoKnownAs: | Immuno20Sproteasome | |||||||||||||||
CatalogNo.: | A1501 | |||||||||||||||
Size: | 50µg | |||||||||||||||
MolecularWeight: | 700kDa | |||||||||||||||
Species: | Bovine | |||||||||||||||
Source: | Bovineredbloodcells | |||||||||||||||
Stock: | 20mMTris,20mMNaCl,1mMEDTA,5mMβME,10%Glycerol | |||||||||||||||
Concentration: | Seetubelabel | |||||||||||||||
QualityAssurance: | ~95%bynative-PAGE,seedatasheetforgelimage | |||||||||||||||
Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles | |||||||||||||||
PDFDataSheet: | PDFDatasheet,MSDS | |||||||||||||||
NCBIRefSeq: | N/A | |||||||||||||||
Image(s): | (Clickimagetoenlarge)
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ShippingMethod: | Dryiceshipping | |||||||||||||||
References: | 1.GoldbergAL,etal.(1992)Nature357(6377),375–379. 2.AkiM,etal.(1994)JBiochem115(2),257–269.3.TanakaK(1994)JLeukocBiol56(5),571–575. 4.ZaissDM,etal.(2011)JImmunol187(5),2302–2309. |
Coomassie-stainedSDS-PAGE Lane1:MolecularweightmarkersLane2:5µgpurifiedBovineimmuno20Sproteasome | Coomassie-stainednative-PAGE Lane1:5µgpurifiedBovineimmuno20Sproteasome | |
Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28α(Cat.#A2100),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively. | Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28β(Cat.#A2200),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively. | Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28γ(Cat.#A2300),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively. |
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