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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ubpbio/Bovine immuno 20S proteasome/A1501/50 µg
产品编号:A1501
市  场 价:¥6400.00
场      地:美国(厂家直采)
产品分类: 蛋白类>>蛋白酶类>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$320.00
品      牌: UBPBio
公司分类:
ubpbio/Bovine immuno 20S proteasome/A1501/50 µg
商品介绍
UponstimulationwithIFN–γ,theexpressionofthethreecatalyticβsubunitsβ1,β2,andβ5withiso-formsβ1i(LMP2),β2i(MECL–1),andβ5i(LMP7)areinduced,respectively.Thesesubunitsareincorporatedintothe20Sproteasometoformtheimmune20Sproteasome.Itwasreportedthattheimmunoproteasomehasalteredproteolyticactivitiescomparedtoitsnormalform,whichfavorthegenerationofimmunopeptidesforantigenpresentation.

AdditionalInformation

Details

UponstimulationwithIFN–γ,theexpressionofthethreecatalyticβsubunitsβ1,β2,andβ5withiso-formsβ1i(LMP2),β2i(MECL–1),andβ5i(LMP7)areinduced,respectively.Thesesubunitsareincorporatedintothe20Sproteasometoformtheimmune20Sproteasome.Itwasreportedthattheimmunoproteasomehasalteredproteolyticactivitiescomparedtoitsnormalform,whichfavorthegenerationofimmunopeptidesforantigenpresentation.
Images:(Clickimagetoenlarge)
ProductName:Bovineimmuno20Sproteasome
AlsoKnownAs:Immuno20Sproteasome
CatalogNo.:A1501
Size:50µg
MolecularWeight:700kDa
Species:Bovine
Source:Bovineredbloodcells
Stock:20mMTris,20mMNaCl,1mMEDTA,5mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:~95%bynative-PAGE,seedatasheetforgelimage
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFDatasheetMSDS
NCBIRefSeq:N/A
Image(s):(Clickimagetoenlarge)
Coomassie-stainedSDS-PAGE
Lane1:MolecularweightMarkersLane2:5µgpurifiedBovineimmuno20Sproteasome
Coomassie-stainednative-PAGE
Lane1:5µgpurifiedBovineimmuno20Sproteasome



Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28α(Cat.#A2100),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28β(Cat.#A2200),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28γ(Cat.#A2300),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.
ShippingMethod:Dryiceshipping
References:1.GoldbergAL,etal.(1992)Nature357(6377),375–379.
2.AkiM,etal.(1994)JBiochem115(2),257–269.3.TanakaK(1994)JLeukocBiol56(5),571–575.
4.ZaissDM,etal.(2011)JImmunol187(5),2302–2309.
Coomassie-stainedSDS-PAGE
Lane1:MolecularweightmarkersLane2:5µgpurifiedBovineimmuno20Sproteasome
Coomassie-stainednative-PAGE
Lane1:5µgpurifiedBovineimmuno20Sproteasome



Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28α(Cat.#A2100),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28β(Cat.#A2200),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28γ(Cat.#A2300),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.
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