ubpbio/Bovine 20S proteasome/A1401/50 µg,UBPBio,,UBPBio,Ubiquitin-Proteasome Biotechnologies,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ubpbio/Bovine 20S proteasome/A1401/50 µg

产品编号：A1401

市场价：¥4500.00

场地：美国(厂家直采)

产品分类：蛋白类>酶>蛋白酶类>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$225.00

品牌： UBPBio

公司：Ubiquitin-Proteasome Biotechnologies, LLC

公司分类：

商品介绍

The20Sproteasomehasabarrel–shapedstructurearrangedasfourheptomericringsofαββα.Ineukaryotes,eachofαandβringiscomposedofsevendifferentproteins.Theβ1,β2andβ5subunitshave‘caspase-like’,‘trypsin-like’and‘chymotypsin-like’activities,respectively.In26Sproteasome-mediatedproteindegradation,toentrytheβchamberofthe20Sproteasomethathousestheproteolyticsites,asubstrateproteinhastopassthroughasubstratetranslocationchannelconsistingofthedouble-ringformedbysixATPasesofPA700andtheαchamberformedbyαsubunitsofthe20Sproteasome.

AdditionalInformation

Details

ProductName:

Bovine20Sproteasome

AlsoKnownAs:

20Sproteasome

CatalogNo.:

A1401

Size:

50µg

MolecularWeight:

700kDa

Species:

Bovine

Source:

Bovineredbloodcells

Stock:

20mMTris,20mMNaCl,1mMEDTA,5mMβME,10%Glycerol

Concentration:

Seetubelabel

QualityAssurance:

~95%bynative-PAGE,seedatasheetforgelimage

Storage:

Storeat-80°C;avoidmultiplefreeze-thawcycles

PDFDataSheet:

PDFDatasheet，MSDS

NCBIRefSeq:

N/A

Image(s):


Coomassie-stainednative-PAGE Lane1:5µgpurifiedBovine20Sproteasome	Coomassie-stainednative-PAGE Lane1:5µgpurifiedBovine20Sproteasome


Activationof5nM20Sproteasomeby25nMPA700(Cat.#A1300),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.	Activationof5nM20Sproteasomeby25nMPA28α(Cat.#A2100),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.	Activationof5nM20Sproteasome(Cat.#A1400)by25nMPA28β(Cat.#A2200),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.

ShippingMethod:

Dryiceshipping

References:

1.WaxmanL,etal.(1987)JBiolChem262(6),2451–2457.
2.GanothD,etal.(1988)JBiolChem263(25),12412–12419.3.CouxO,etal.(1996)AnnuRevBiochem65,801–847.
4.KimHM,etal.(2011)BiochimicaBiophysicaActa1809(2),67–79.


Coomassie-stainednative-PAGE Lane1:5µgpurifiedBovine20Sproteasome	Coomassie-stainednative-PAGE Lane1:5µgpurifiedBovine20Sproteasome


Activationof5nM20Sproteasomeby25nMPA700(Cat.#A1300),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.	Activationof5nM20Sproteasomeby25nMPA28α(Cat.#A2100),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.	Activationof5nM20Sproteasome(Cat.#A1400)by25nMPA28β(Cat.#A2200),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.

品牌介绍

泛素-蛋白酶体生物技术有限公司（UBPBio），LLC是一家私有公司，由科学家和投资人于2011年成立。UBPBio致力于为学术界和工业界提供高质量的试剂，以加快对泛素-蛋白酶体系统及相关途径的发现。。目前，我们的产品线包括16个类别，提供350多种产品。如果您在泛素领域需要特殊试剂，请与我们联系，我们将竭诚为您服务。

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