ProductName: | Bovine19S(PA700) | ||||||||
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AlsoKnownAs: | No | ||||||||
CatalogNo.: | A1300 | ||||||||
Size: | 25µg | ||||||||
MolecularWeight: | 900kDa | ||||||||
Species: | Bovine | ||||||||
Source: | Bovineredbloodcells | ||||||||
Stock: | 20mMTris,20mMNaCl,ImMEDTA,5mMβME,10%Glycerol | ||||||||
Concentration: | Seetubelabel | ||||||||
QualityAssurance: | Atleast90%bynative-PAGE,seedatasheetforgelimage | ||||||||
Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles | ||||||||
PDFDataSheet: | PDFDatasheet,MSDS | ||||||||
NCBIRefSeq: | N/A | ||||||||
Image(s): | (Clickimagetoenlarge)
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ShippingMethod: | Dryiceshipping | ||||||||
References: | 1.WaxmanL,etal.(1987)JBiolChem262(6),2451–2457. 2.KanayamaHO,etal.(1992)EurJBiochem206(2),567–578.3.GlickmanMH,etal.(1998)Cell94,615–623. 4.DaFonsecaPC,etal.(2008)JBiolChem283,23305–23314.5.KimHM,etal.(2011)BiochimBiophysicaActa1809(2),67–79. |
Coomassie-stainedSDS-PAGE Lane1:MolecularweightmarkersLane2:5µgpurifiedBovine19S(PA700) |
Coomassie-stainednative-PAGE Lane1:5µgpurifiedBovine19S(PA700) |
Activationof5nM20Sproteasome(Cat.#A1400)by25nMPA700,theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively. |
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